Alternative pre-mRNA Splicing: Theory and Protocols by Stefan Stamm, Chris Smith, Reinhard Lührmann PDF

By Stefan Stamm, Chris Smith, Reinhard Lührmann

ISBN-10: 3527326065

ISBN-13: 9783527326068

ISBN-10: 3527636773

ISBN-13: 9783527636778

The publication involves significant elements: The first one presents a short theoretical
introduction that offers a brief review of other splicing and cites key papers within the field for extra in-depth details. the second one half is a suite of experimental protocols which are utilized in the field of different splicing.
Contents
1 Splicing within the RNA World
2 RNPs, Small RNAs, and miRNAs
3 RNA components occupied with Splicing
4 A Structural Biology point of view of Proteins all for Splicing Regulation
5 The Spliceosome in Constitutive Splicing
6 using Saccharomyces cerevisiae to check the Mechanism of pre-mRNA Splicing
7 demanding situations in Plant substitute Splicing
8 replacement Splice website Selection
9 Integration of Splicing with Nuclear and mobile Events
10 Splicing and Disease
11 From Bedside to Bench: how one can study a Splicing Mutation
12 research of universal Splicing Problems
13 Ultracentrifugation within the research and Purification of Spliceosomes Assembled In Vitro
14 Chemical Synthesis of RNA
15 RNA Interference (siRNA, shRNA)
16 Expression and Purification of Splicing Proteins
17 Detection of RNA–Protein Complexes by means of Electrophoretic Mobility Shift Assay
18 useful research of enormous Exonic Sequences via Iterative In Vivo Selection
19 id of Splicing cis-Elements via an Ultra-Refined Antisense Microwalk
20 Genomic SELEX to spot RNA ambitions of Plant RNA-Binding Proteins
21 Quantification of other Splice Variants
22 High-Throughput research of different Splicing by means of RT-PCR
23 tracking alterations in Plant substitute Splicing Events
24 Array Analysis
25 The CLIP way to learn Protein–RNA Interactions in Intact Cells and Tissues
26 RNA–Protein Crosslinking and Immunoprecipitation (CLIP) in Schizosaccharomyces pombe
27 id of Proteins guaranteed to RNA
28 Single-Cell Detection of Splicing occasions with Fluorescent Splicing Reporters
29 The guidance of HeLa mobile Nuclear Extracts
30 In Vitro Splicing Assays
31 meeting and Isolation of Spliceosomal Complexes In Vitro
32 research of Site-Specific RNA–Protein Interactions
33 Immunoprecipitation and Pull-Down of Nuclear Proteins
34 research of Protein (-RNA) Complexes through (Quantitative) Mass Spectrometric Analysis
35 quickly Cloning of Splicing Reporter Minigenes
36 In Vivo research of Splicing Assays
37 Coupled Promoter Splicing Systems
38 strong telephone strains with Splicing Reporters
39 Splicing issue ChIP and ChRIP: Detection ofSplicing and Splicing components at
Genes via Chromatin Immunoprecipitation
40 Yeast Genetics to enquire the functionality of middle Pre-mRNA Splicing Factors
41 research of HIV-1 RNA Splicing
42 In Vivo research of Plant Intron Splicing
43 amendment State-Specific Antibodies
44 research of other Splicing in Drosophila Genetic Mosaics
45 Antisense Derivatives of U7 Small Nuclear RNA as Modulators of Pre-mRNA Splicing
46 Screening for replacement Splicing Modulators
47 Use of Oligonucleotides to alter Splicing
48 altering indications to the Spliceosome
49 review of Splicing suitable Databases
50 research of RNA Transcripts by way of High-Throughput RNA Sequencing
51 id of Splicing issue objective Genes via High-Throughput Sequencing
52 Bioinformatic research of Splicing Events
53 research of Pre-mRNA Secondary constructions and substitute Splicing
54 constitution Prediction for however Spliced Proteins
55 Comparative Genomics tools for the Prediction of Small RNA-Binding websites

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Additional info for Alternative pre-mRNA Splicing: Theory and Protocols

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Mol. Cell, 8, 375–381. M. (2008) Disease mechanism for 12 43 44 45 46 j 1 Splicing in the RNA World retinitis pigmentosa (RP11) caused by missense mutations in the splicing factor gene PRPF31. Mol. , 14, 683–690. D. (2007) prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast. Nat. Struct. Mol. , 14, 1077–1083. T. et al. (2002) Mutations in HPRP3, a third member of pre-mRNA splicing factor genes, implicated in autosomal dominant retinitis pigmentosa.

Perutz Laboratories Dr. Bohrgasse 9/3 1030 Vienna Austria Diana Baralle University of Southampton Human Genetics Division Duthie Building (Mailpoint 808) Southampton General Hospital Tremona Road Southampton SO16 6YD UK Francisco E. Baralle International Centre of Genetic Engineering and Biotechnology (ICGEB) Department of Molecular Pathology Padriciano 99 34012 Trieste Italy Marco Baralle International Center of Genetic Engineering and Biotechnology (ICGEB) Department of Molecular Pathology Padriciano 99 34012 Trieste Italy Jean D.

RNA is processed into smaller RNA fragments that can have functions. 1 Introduction The complete sequencing of the human genome revealed that only about 2% of the genetic information is covered by protein-coding genes [1]. Interestingly, less-complex eukaryotes encode for a similar number of proteins, and it is very likely therefore that non-protein-coding genomic regions contribute significantly to the complexity of higher eukaryotes. Gene expression analyses have revealed that active transcription is not restricted to protein-coding genes, but includes different classes of non-proteincoding RNAs.

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Alternative pre-mRNA Splicing: Theory and Protocols by Stefan Stamm, Chris Smith, Reinhard Lührmann


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